American Society of Hematology

Fig. 4.

$Fig. 4. Arp2/3 complex incorporation into the platelet cytoskeleton. / (A) WAS platelets were activated with 25 μM TRAP (●) or 3 μg/mL CRP (○) for the indicated times. (B) Wild-type (░) and WASp-deficient (■) mouse platelets were activated with 1 U/mL thrombin for 1 minute. F-actin–associated Arp2/3 complex was collected by centrifugation of Triton X-100 platelet lysates at 100 000g for 30 minutes at 4°C. F-actin–associated and soluble fractions were displayed by SDS-PAGE, transferred onto an Immobilon-P membrane, and probed with a rabbit polyclonal antibody directed against Arp3. The amount of Arp2/3 complex in the cytoskeletal fraction relative to total was quantified by densitometric analysis of the immunoblots. Values are mean of 3 to 4 experiments ± SD.$

Arp2/3 complex incorporation into the platelet cytoskeleton.

(A) WAS platelets were activated with 25 μM TRAP (●) or 3 μg/mL CRP (○) for the indicated times. (B) Wild-type (░) and WASp-deficient (■) mouse platelets were activated with 1 U/mL thrombin for 1 minute. F-actin–associated Arp2/3 complex was collected by centrifugation of Triton X-100 platelet lysates at 100 000g for 30 minutes at 4°C. F-actin–associated and soluble fractions were displayed by SDS-PAGE, transferred onto an Immobilon-P membrane, and probed with a rabbit polyclonal antibody directed against Arp3. The amount of Arp2/3 complex in the cytoskeletal fraction relative to total was quantified by densitometric analysis of the immunoblots. Values are mean of 3 to 4 experiments ± SD.

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