Expression of active caspase in cells with trisomy 8.

Freshly obtained BM smears from patients with trisomy 8 were stained for activated caspase as described in “Patients, materials, and methods,” then subsequently subjected to FISH for chromosome 8. Cells were scored for caspase and karyotype. The histograms show increased caspase staining in cells positive for trisomy 8 when compared with cells with a normal karyotype from the same patient. Normal BM demonstrated less than 3% caspase staining.