Effect of Delta4 expression on lymphoproliferative disease.

Delta4 expression causes severe lymphoproliferative disease. Animals were killed at week 15, and tissues were collected for histology and FACS analysis; MSCVpac, n = 4; MigR, n = 2; MSCVpacDelta4, n = 2; MigRDelta4, n = 2. (A) The mean values ± SD for total body and organ weights are shown. (B) Representative paraffin sections stained with hematoxylin and eosin are shown for bone marrow, spleen, lung, intestine, and kidney from control animals and animals transduced with Delta4. Magnification × 200. (C) Single-cell suspensions were prepared from bone marrow, spleen, and thymus and stained with the antibodies indicated. Mean values (± SD) for the numbers of positive cells are given with the use of gating as shown in Figure3A.