eGFP expression in human CD4⁺ and CD8⁺ T cells transduced with the LvCD4 and LvCD4-Sil vectors in vitro.

PHA- or IL-7–activated total human lymphocytes were transduced with the LvCD4 (upper panels) and the LvCD4-Sil (lower panels) vectors as described in “Materials and methods.” Cells were stained with monoclonal antibodies against human CD4 and CD8 and were analyzed by 3-color flow cytometry. Shown are the typical profiles of eGFP expression 4 days after transduction in gated CD4⁺ (left panels) and CD8⁺ (right panels) T cells. MFI indicates mean fluorescence intensity of eGFP⁺ cells. Dotted lines represent background of eGFP expression in nontransduced similar subsets of lymphocytes.