Video analysis of leukocytes at the site of inflammation.

PRE indicates analysis performed before surgery, and INF indicates analysis 24 hours after surgery when the inflammatory reaction was most vivid. Patients without hydrocortisone treatment are marked with blue bars and patients with hydrocortisone treatment with black. Pvalues between the groups were calculated with Mann-WhitneyU test. *P <.05. (A) Number of rolling cells in conjunctival venules was significantly higher in specimens taken during inflammation than in specimens taken before inflammation (groups pooled in the Figure), and hydrocortisone had no effect on this variable. (B) Relative frequencies of leukocyte rolling velocities in specimens taken during the inflammation, with or without preoperative hydrocortisone treatment. Leukocyte rolling in the nontreated patients was significantly slower than in the hydrocortisone-treated patients. (C) Number of extravasated leukocytes per square millimeter in conjunctival tissue. Specimens taken before the inflammation contained essentially no leukocytes, but the number was significantly elevated in the specimens taken during the inflammation versus those taken before the inflammation, and hydrocortisone treatment significantly reduced their numbers.