Analyses of endothelial expression of P-selectin and sulfated sLex glycans on L-selectin ligands in conjunctiva.

The percentage of conjunctival venules expressing P-selectin (A); MECA-79, reactive with sulfated extended core 1 mucin–type O-glycans (E); 2F3 detecting sLex epitopes in the specimens taken before and during the inflammation without (blue bars) and with (black bars) hydrocortisone pretreatment (I). P values between the groups were calculated with Mann-Whitney U test. *P < .05. (B) (F) (J) Isotype-matched background controls visible with essentially no reactivity. (C) (G) (K) Staining of specimens taken before the inflammation where a small proportion of vessels react with anti–P-selectin antibodies (brown in panel C), but no MECA-79 reactivity is recorded. The 2F3 stains epithelial sLex, but the endothelium lining the vessels is negative. (D) (H) (L) Staining of specimens taken during the inflammation for endothelial P-selectin, MECA-79, and 2F3 with reactive endothelium within vessels (red-brown) is visible in all panels. Original magnification, × 200.