Fig. 6.
Fig. 6. IVIG effects on the 7E3-platelet binding curve are essentially superimposed. / Because the 2 lines lie very close to each other, the 2 lines appear as 1 solid line in the figure. Total platelet concentration was held constant as the 7E3 concentration was increased, in the presence (○) or absence (▿) of IVIG. Free (ie, unbound) 7E3 concentrations were determined by ELISA. Data were fit as described in the text. The lines represent the best fits of the data sets (solid line indicates IVIG, broken line, no IVIG) and are essentially superimposed. Parameters (KA and Rt) obtained from the fits did not differ significantly. Without IVIG present, KA was 4.9 ± 0.7 × 108 M−1and Rtwas 7.5 ± 0.4 × 10−8 M (55 000 ± 3000 GP/platelet). With IVIG, KA was 5.5 ± 1.2 × 108 M−1 and Rtwas 7.6 ± 0.7 × 10−8 M (56 000 ± 5000 GP/platelet). IVIG does not prevent 7E3 from binding to platelets.

IVIG effects on the 7E3-platelet binding curve are essentially superimposed.

Because the 2 lines lie very close to each other, the 2 lines appear as 1 solid line in the figure. Total platelet concentration was held constant as the 7E3 concentration was increased, in the presence (○) or absence (▿) of IVIG. Free (ie, unbound) 7E3 concentrations were determined by ELISA. Data were fit as described in the text. The lines represent the best fits of the data sets (solid line indicates IVIG, broken line, no IVIG) and are essentially superimposed. Parameters (KA and Rt) obtained from the fits did not differ significantly. Without IVIG present, KA was 4.9 ± 0.7 × 108 M−1and Rtwas 7.5 ± 0.4 × 10−8 M (55 000 ± 3000 GP/platelet). With IVIG, KA was 5.5 ± 1.2 × 108 M−1 and Rtwas 7.6 ± 0.7 × 10−8 M (56 000 ± 5000 GP/platelet). IVIG does not prevent 7E3 from binding to platelets.

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