Survival analysis following total neutrophil depletion.

The depletion of neutrophils abrogated the protective response againstA fumigatus demonstrated by the myeloid progenitors (CMP/GMP). (A) On D+5, anti–Gr-1 (1 mg) was given to mice that underwent transplantation with HSCs (gray filled triangles) or HSC+CMP/GMP (black filled squares). The anti–Gr-1 treatment groups were infected on D+7 along with a group of untreated mice that underwent transplantation with HSCs (gray filled circles). The survival of mice that underwent transplantation with HSCs+CMP/GMP and were then treated with anti–Gr-1 was reduced to equivalence to animals that underwent transplantation with HSCs only as opposed to their nondepleted counterparts, which have demonstrated a survival rate of 67% in this series of experiments. Arrows represent anti–Gr-1 administration and A fumigatus challenge. An HSC-only transplantation group (gray open circles) served as control for hematopoietic reconstitution. (B) FACS analysis of splenocytes from mice that underwent transplantation with HSCs only or HSC+CMP/GMP. To assess the extent of neutrophil depletion, an uninfected representative animal from each group was treated with anti–Gr-1 on D+5 and analyzed on D+7. The values indicate the percentage of neutrophils (Mac-1⁺Gr-1⁺) of total live splenocytes.