Contemporaneous images representing the bioluminescence of mice 18 hours after intraperitoneal inoculation of 300 CFUs ofP aeruginosa XEN5.

This strain of Pseudomonas constitutively expresses a bacterial luciferase and the enzymes necessary for the substrate biosynthesis from the lux operon. The color scale represents bioluminescence intensity (photon counts) in the images as measured using IVIS. Mice that received grafts containing only HSCs (A) demonstrated a strong bioluminescent signal that contrasts the lack of signal in the infected mice that received a graft containing HSCs and CMP/GMP. Rapid dissemination of P aeruginosa occurred in mice that received HSC-only grafts. In contrast, cotransplantation with CMP/GMP prevented bacterial dissemination and subsequent morbidity as evidenced by the bioluminescent images (B) and CFU determination.