Aspergillus -specific T-cell responses in healthy volunteers.

Aspergillus-specific T-cell responses were assessed by a lymphoproliferation assay. A stimulation index of 3 or higher was considered to indicate a positive lymphoproliferative T-cell response.Aspergillus-specific T-cell proliferation was detectable against EC SAB in 14 of 16 healthy individuals, against heat-inactivated conidia in 7 of 8, and against 2 recombinant A fumigatus proteins expressed in P pastoris, the 90-kDa catalase in 8 of 11 and the 88-kDa DPP V in 4 of 16, respectively. EC SAB indicates cellular extracts of A fumigatus; Cat19, 90-kDa catalase; and DPP, dipeptidylpeptidase.