Aspergillus -specific T-cell stimulation in healthy volunteers is associated with increased production of IFN-γ and IL-10 in culture supernatants.

Cytokines were measured by standard ELISAs in the culture supernatants of PBMNCs stimulated with A fumigatus proteins. After stimulation of PBMNCs with EC SAB, an increase in the production of IFN-γ (median, 128.7 pg/mL; range, 21.3-1519 pg/mL) and IL-10 (median, 39.4 pg/mL; range, 1.5-899.4 pg/mL) was documented. The cytokine release in culture supernatants after stimulation with the 90-kDa catalase revealed comparable results (median IFN-γ concentration, 149.3 pg/mL; range, 14.4-> 2000 pg/mL; median IL-10 concentration, 10.05 pg/mL; range, 3.6-168.5 pg/mL). EC SAB indicates cellular extracts of A fumigatus; Cat19, 90-kDa catalase; ▿, IFN-γ; ▵, IL-10; and *, IL-10 concentrations were assessed in 10 of 11 healthy individuals.