Phosphoantigen-reactive Vγ9/Vδ2 T-cell clones, but not α/β, and phosphoantigen-nonreactive γ/δ T-cell clones, produce TNF in response to A fumigatus antigens.

Aspergillus-specific T-cell responses were assessed by a TNF bioassay using the ultrasensitive WEHI-164 clone 13. The phosphoantigen-reactive Vγ9/Vδ2 T-cell clones C49 and 6E1, but not the phosphoantigen-nonreactive γ/δ T-cell clone AB18 and the αβ T-cell clone B1, produced significant amounts of TNF in response toA fumigatus antigen EC SAB.