Comparative analysis of Aspergillus-, tetanus toxoid–, and CMV antigen–specific T-cell responses in patients late after allogeneic SCT.

Cytokines were measured by standard ELISAs in the culture supernatants of PBMNCs stimulated with A fumigatus antigen EC SAB. Panel A shows the stimulation indices, and panel B the ratio of IFN-γ and IL-10. An SI of 3 or higher was considered to indicate a positive lymphoproliferative T-cell response. Aspergillus-specific T-cell responses in patients late after allogeneic SCT were characterized by a low SI and a low IFN-γ/IL-10 ratio.