Functional correction of oxidase activity in cultured X-CGD CD34⁺PBSCs under optimum conditions for ex vivo transduction with lentivector or oncoretrovirus vector.

Shown is the correction of oxidase activity over 28 days (after last transduction) of culture of X-CGD CD34⁺PBSCs under growth-stimulating conditions (5 cytokines) comparing nontransduced control X-CGD CD34⁺PBSCs (■) with the same cells that had been subjected to 3 daily 7-hour transductions using third-generation SIN lentivector–gp91^phox (○) or MFGS–gp91^phox (♦). PMA-stimulated superoxide generation, as measured in a chemiluminescence assay, is expressed as the percentage of the stimulated superoxide generation by a similar culture of normal CD34⁺PBSCs. Results are representative of 2 experiments.