Flow cytometric analyses of high side scatter human CD45⁺ cells in the bone marrow of NOD/SCID mice that underwent transplantation with human CD34⁺PBSCs.

Shown are representative dot plots of analysis of bone marrow cells from (A) a NOD/SCID mouse that had not received any transplant and from NOD/SCID mice that received transplants of (B) 4-day cultured but nontransduced X-CGD CD34⁺PBSCs, (C) lentivector–gp91^phox-transduced X-CGD CD34⁺PBSCs, and (D) lentivector–eGFP-transduced normal CD34⁺PBSCs. Side scatter is plotted on the vertical axis, and labeling with Per-CP–conjugated antihuman CD45 antibody is plotted on the horizontal axis. Because some of the plots are from experiments analyzed on different days, it demonstrates the analysis variability seen in the apparent separation of the CD45⁺ cells from the negative cells, in which the boxed areas enclosed the CD45⁺cells. All NOD/SCID mice that underwent transplantation and were analyzed for this study (Figures 6, 8, 9) had at least 15% CD45⁺ cells in the chimeric bone marrow as determined by the type of analysis shown in this figure.