Flow cytometric analyses of the expression of eGFP by high side scatter human CD45⁺ cells in the bone marrow of NOD/SCID mice that underwent transplantation with normal human CD34⁺PBSCs transduced with lentivector–eGFP.

Shown are representative dot plots of analyses of eGFP expression in human (CD45⁺) cells engrafted in chimeric bone marrow from NOD/SCID mice that underwent transplantation with (A) 4-day cultured but nontransduced normal CD34⁺PBSCs and (B) lentivector–eGFP-transduced normal CD34⁺PBSCs. Side scatter is plotted on the vertical axis, and fluorescence by eGFP is plotted on the horizontal axis. Analyses are gated to include only those cells that label positive for the CD45 antigen, as depicted in Figure 7. Boxed areas in this figure are the events indicating cells that are eGFP positive. There are no such events in panel A but 42% of the human cells are positive in panel B.