Human cell engraftment in NOD/SCID mice receiving transplants of limiting doses of ABM CD34+ cells and HUBEC-cultured progeny.
(A) First, 1 × 105 ABM CD34+ cells (left) or their progeny following HUBEC culture (right) were transplanted into NOD/SCID mice. The level of human cells present in the murine bone marrow at 8 weeks was then determined by flow cytometric analysis of human CD45 expression. Panel B shows 5 × 105 ABM CD34+ cells or their HUBEC-cultured progeny; panel C, 1 × 106 ABM CD34+ cells versus HUBEC-cultured progeny; and panel D, 1.5 × 106 ABM CD34+ cells versus HUBEC-cultured progeny. Panel E shows the engraftment of progeny of 1.5 × 106 ABM CD34+ cells cultured with HUBEC noncontact cultures as well as the engraftment of the progeny of 3 × 106 ABM CD34+ cells cultured with GM-CSF plus IL-3 plus IL-6 plus SCF plus flt-3 ligand in the absence of HUBECs.
