CML-DCs have altered morphology and F-actin distribution.

DCs were allowed to adhere to fibronectin-coated glass coverslips for 4 hours and were then fixed and stained with tetramethyl B rhodamine isothiocyanate (TRITC)–labeled phalloidin to show F-actin. Images are shown of 2 independent preparations of normal DCs (A,D,G, and J) and for CML-DCs from patient 1 (B,H), patient 2 (C,I), patient 3 (E,K), and patient 6 (F,L). Low-magnification images (A-F) were collected with a cooled charge-coupled device (CCD) camera (scale bar in panel F represents 100 μm) and high-magnification images (G-L) with a confocal laser scanning microscope (scale bar in panel L represents 20 μm). Arrows indicate regions within cells that contain clusters of podosomes.