CML-DCs are defective in antigen processing.

DCs were generated from healthy individuals (○) and from CML patients (●): patient 1 (A,D), patient 2 (B,E), and patient 7 (C,F). Immature HLA-DR7–expressing DCs were pulsed overnight with either whole TT (0.008 IU/mL) (A-C) and HA307-319 peptide (10 μg/mL) (D-E) or TT947-967 peptide (10 μg/mL) (F). Different numbers of irradiated and prepulsed DCs were cultured in the presence of either TT-specific T-cell clones ([A] clone TT1; [B] clone TT1, solid lines, and clone CG1, dotted lines; [C] clone JNI27) or HA307-319–specific T-cell clone ([D-E] clone 7P61) or TT947-967–specific T-cell clone ([F] clone JNI27). After 2 days, ³HTdR was added and thymidine incorporation was measured after 20 hours. The data are expressed as counts per minute (cpm) ± standard deviations (SD). Results are representative of 15 experiments obtained with DCs derived from different patients as indicated in Table 1.