CML-DCs derived from CD34⁺ cells are defective in antigen processing.

DCs were generated from healthy individuals (open symbols) and from CML patients (filled symbols). DCs were obtained from the adherent population of PBMCs (circles) or from CD34⁺ cells (squares and triangles): patient 1 (A-B); patients 7 and 8 (C-D). HLA-DR7–expressing DCs were pulsed overnight with whole TT (0.008 IU/mL) (A,C) and HA307-319 peptide (10 μg/mL) (B,D). Different numbers of irradiated and prepulsed DCs were cultured in the presence of either TT-specific (A,C) or HA307-319–specific T-cell clones (B,D). After 2 days, ³HTdR was added and thymidine incorporation was measured after 20 hours. The data are expressed as counts per minute (cpm) ± standard deviations (SD). The results are representative of 3 experiments.