Tax activation of the Fuc-T VII promoter is dependent on the CRE-like element and on its flanking G+C–rich sequences.

(A) The CRE-like element and the luciferase reporter construct are represented by closed and open boxes, respectively. The Fuc-T VIIpromoter from nucleotide −791 to +104 with the CRE-like element underlined. Specific mutations in each reporter construct are shown below. (B) Cells were transfected with 4 μg mutant reporter construct alone or in combination with 4 μg pCGTax. Results are expressed as relative light units normalized for CAT activity. Error bars depict the standard deviations from 3 independent experiments.