Inhibition of the mitochondrial import of preCPO by high hemin concentration.

Import reaction was performed in the presence or absence of hemin at the indicated concentrations for 30 minutes at 30°C. Then mitochondria were treated with trypsin and CPO, and Sub9-DHFR and AAC were analyzed by fluorography. The bands of mature CPO, mature Sub9-DHFR, and AAC were quantified by densitometry of exposed film. ● represents CPO; ▧, Sub9-DHFR; and ▴, AAC.