Flow cytometric analysis of soluble fibrinogen binding to the cell surface-expressed GPIIb-Ala435IIIa.

Soluble fibrinogen was labeled with FITC as described in “Materials and methods.” Transfected CHO cells were incubated for 45 minutes at 22°C with either FITC-labeled fibrinogen or FITC-labeled BSA at a concentration of 100 μg/mL in the presence or absence of RGDW peptide (2 mM), followed by flow cytometric analysis. The left panel showed fibrinogen binding to WT GPIIb-IIIa on CHO cells, and the right panel showed the binding of soluble FITC-conjugated fibrinogen to GPIIb-Ala435IIIa on the transfected CHO cells (top). The bottom panel showed the data of blocking experiments using RGDW peptide.