Fig. 5. Flow cytometric analysis of soluble fibrinogen binding to the cell surface-expressed GPIIb-Ala435IIIa. / Soluble fibrinogen was labeled with FITC as described in “Materials and methods.” Transfected CHO cells were incubated for 45 minutes at 22°C with either FITC-labeled fibrinogen or FITC-labeled BSA at a concentration of 100 μg/mL in the presence or absence of RGDW peptide (2 mM), followed by flow cytometric analysis. The left panel showed fibrinogen binding to WT GPIIb-IIIa on CHO cells, and the right panel showed the binding of soluble FITC-conjugated fibrinogen to GPIIb-Ala435IIIa on the transfected CHO cells (top). The bottom panel showed the data of blocking experiments using RGDW peptide.
Fig. 5.

Flow cytometric analysis of soluble fibrinogen binding to the cell surface-expressed GPIIb-Ala435IIIa.

Soluble fibrinogen was labeled with FITC as described in “Materials and methods.” Transfected CHO cells were incubated for 45 minutes at 22°C with either FITC-labeled fibrinogen or FITC-labeled BSA at a concentration of 100 μg/mL in the presence or absence of RGDW peptide (2 mM), followed by flow cytometric analysis. The left panel showed fibrinogen binding to WT GPIIb-IIIa on CHO cells, and the right panel showed the binding of soluble FITC-conjugated fibrinogen to GPIIb-Ala435IIIa on the transfected CHO cells (top). The bottom panel showed the data of blocking experiments using RGDW peptide.

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