MIP-1α induces proliferation of MM cell lines, which can be inhibited by MEK1 inhibitor PD 98059.

(A) MM1.S, (B) pat MM, (C) H929, (D) INA-6, and (E) OPM2 cells were incubated for 48 hours without or with MIP-1α (100 ng/mL). Data represent means ± SDs for at least triplicate samples. Statistical significance is for MM1.S, P = .010; pat MM,P = .000 28; H929, P = .0008; INA-6,P < .01; and OPM2, P = .0007. (F) MM1.S and pat MM cells were incubated for 48 hours with or without MIP-1α (500 ng/mL). Untreated and treated MM cells were incubated with MEK1 inhibitor PD 98059 (50 μM). Incubation with MIP-1α led to a significant increase of proliferation (MM1.S, P = .03; pat MM, P = .0002) that could be abrogated by PD 98059 treatment. Data represent means ± SDs for triplicate samples.