bFGF expression in laser-microdissected megakaryocytes.

In microdissected megakaryocytes, overexpression of bFGF mRNA was found in PV (× 10, P < .0001), in fibrotic IMF (× 8.9,P < .001), in prefibrotic IMF (× 6.8,P < .009), and in ET (× 6.8, P < .0001), compared to control megakaryocytes. In CML, megakaryocytes exhibited moderately higher bFGF mRNA levels compared to controls without significance, but significant differences occurred in comparison to PV (P < .0001), fibrotic IMF (P < .002), and prefibrotic IMF and ET (P < .04 andP < .001, respectively). Real-time RT-PCR with bFGF forward primer 5′-CCCGGCCACTTCAAGGA; bFGF reverse primer 5′-TGGATGCGCAGGAAGAAG and bFGF probe 5′-CCCAAGCGGCTGTACTGCAAAAACG. β-Glucuronidase (β-GUS) forward primer 5′-CTCATTTGGAATTTTGCCGATT, β-GUS reverse primer 5′-AGTGAAGATCCCCTTTTTA, and β-GUS probe 5′-TGAACAGTCACCGACGAGAGTGCTGG. Transcript levels are depicted as multiples of the amount found in reactive control cases ( = 1).