Fig. 1.
P-selectin and E-selectin bind to murine WEHI-3 cells, human HL-60 cells, and murine and human neutrophils.
Cell lines (A) or neutrophils (B) were incubated with saturating concentrations of murine P-selectin or E-selectin IgM chimera or control CD45 IgM chimera. Bound chimera was detected by incubation with FITC-conjugated goat anti–human IgM, followed by flow cytometry. Some cells were pretreated with pronase. The data are representative of at least 10 experiments.