Fig. 3.
mAbs to sLex or Lex bind differently to immunoblots of proteins from WEHI-3 and HL-60 cells.
Cell extracts were resolved by SDS-PAGE, transferred to membranes, and probed with the indicated mAb. Bound mAb was identified by peroxidase-conjugated goat anti–murine IgM coupled to a chemiluminescence detection procedure. The data are representative of at least 6 experiments. DF indicates dye front.