Sialidase, chlorate, or α-galactosidase treatment of WEHI-3 cells does not expose epitopes for mAbs to Le^x or sLe^x.

Cells were untreated (control) or pretreated with sialidase, chlorate, both sialidase and chlorate, or α-galactosidase. (A) The cells were incubated with a nonbinding control IgM or with anti-sLe^xmAb CSLEX-1 or anti-Le^x mAb MMA. Bound mAb was detected with FITC-conjugated goat F(ab)′2 fragments to murine IgM. (B) The cells were incubated with murine P- or E-selectin IgM chimera, followed by FITC-conjugated goat anti–human IgM. (C) The cells were incubated with FITC-conjugated GSI-B4 lectin, which recognizes α1-3–linked galactose residues, or with a nonbinding control IgM or anti-Le^x mAb MMA. The data are representative of at least 4 experiments.