Fig. 5.
mAbs to sLex and Lex bind at very low levels to murine neutrophils.
Human or murine neutrophils were untreated or pretreated with sialidase. The cells were incubated with a nonbinding control IgM or with anti-sLex mAb HECA-452 or CSLEX-1 or with anti-Lex mAb MMA or HI98. Bound mAb was detected with FITC-conjugated goat F(ab)′2 fragments to murine IgM. The data are representative of at least 3 experiments.