Forced α1-3-fucosylation of murine neutrophils with FTVI and GDP-fucose does not augment cell rolling on P-selectin or E-selectin in shear flow.
(A) Control or FTVI-treated neutrophils were perfused over P-selectin IgM (90 sites/μm2) or E-selectin IgM (140 sites/μm2) at 0.5 or 1 dyn/cm2. After 4 minutes, the accumulated number of rolling cells was quantified. The data represent the mean ± SD of 3 experiments. (B) Control or FTVI-treated cells were allowed to accumulate on E-selectin at 0.5 dyn/cm2, and cell-free buffer was then introduced. Wall shear stress was increased every 30 seconds, and the percentage of remaining adherent cells was determined. The data represent the means ± SDs from 3 experiments. (C-D) Control or FTVI-treated cells were perfused over P- or E-selectin IgM at 0.5 or 4 dyn/cm2. The data represent the means ± SDs for rolling velocities measured for 50 cells from 3 experiments.
