Fig. 2.
PM-R receptor does not efficiently support CFU-e colony formation in the presence of PRL.
Day-12.5 fetal liver hematopoietic progenitors were infected with MSCV retroviruses encoding the various receptors (P-R, PE-R, and PM-R). (A) 5 × 104 cells were cultured in duplicate in methylcellulose medium with 500 ng/mL ovine prolactin and no EPO. (B) 2.104 cells were cultured with 2 U/mL EPO. Colonies were scored 2 to 3 days later. The result of 1 experiment, representative of 3 independent experiments, is shown.