Enhancement of hematopoietic differentiation of PM-R ES cells in the presence of PRL.

MSCV, P-R, PE-R, and PM-R R1 ES cells were induced to differentiate in the presence of PRL (500 ng/mL) (cocktail PEIS) for 12 days. The hematopoietic progenitor content of EBs was determined by clonogenic assays in methylcellulose, in the presence of 7GF cocktail (1.PEIS/2.7GF) or in the presence of PEIS (1.PEIS/2.PEIS). The results of 2 independent experiments are reported. Types of colonies (CFU): ■, granulocyte-macrophage (-GM); ▤, burst-forming unit-erythroid (BFU-E); ▪, mixed (-Mixt); and ▧, -EMK, or -GMK.