Effect of treatment with forskolin or PGE₁on platelet adhesion to VWF.

Washed human platelets (3 × 10⁸/mL) were perfused for 5 minutes at a shear rate of 150 s^-1 through microcapillaries coated with 1% HSA (⋄) or 25 μg/mL bovine VWF (▪, ■, ░). The cells were pretreated with 10 μM PGE₁ (░), 10 μM forskolin (■), or buffer (▪). Adherent platelets were counted off-line at the indicated times and results are expressed as the mean ± SEM of 2 separate experiments.