Fig. 8.
Fig. 8. Effect of PGE1 on adhesion of K562-GPIb/V/IX cells to VWF. / K562-GPIb/V/IX and K562-GPIb(βSer166Gly)/V/IX cells (1 × 106/mL) were perfused for 10 minutes at a shear rate of 150 s-1 through microcapillaries coated with 1% HSA (♦) or 25 μg/mL bovine VWF (▪,■,▵). The cells were pretreated with 10 μM PGE1 (■), 10 μM adrenaline (▵), or buffer (▪). Adherent cells were counted off-line at the indicated times and results are expressed as the mean ± SEM of 4 separate experiments. PGE1 treatment increased adhesion of K562 cells transfected with the wild-type complex but had no effect on the adhesion of cells expressing the GPIbβ Ser166Gly mutant.

Effect of PGE1 on adhesion of K562-GPIb/V/IX cells to VWF.

K562-GPIb/V/IX and K562-GPIb(βSer166Gly)/V/IX cells (1 × 106/mL) were perfused for 10 minutes at a shear rate of 150 s-1 through microcapillaries coated with 1% HSA (♦) or 25 μg/mL bovine VWF (▪,■,▵). The cells were pretreated with 10 μM PGE1 (■), 10 μM adrenaline (▵), or buffer (▪). Adherent cells were counted off-line at the indicated times and results are expressed as the mean ± SEM of 4 separate experiments. PGE1 treatment increased adhesion of K562 cells transfected with the wild-type complex but had no effect on the adhesion of cells expressing the GPIbβ Ser166Gly mutant.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal