American Society of Hematology

Fig. 3.

$Fig. 3. Kinetics of CD16-induced SHIP-1 translocation in lipid rafts. / Unstimulated (−) or CD16-stimulated cultured NK cells (3 × 108/sample) were fractionated by sucrose gradient centrifugation as described in “Materials and methods.” SHIP-1 immunoprecipitates were obtained from equal amounts of proteins from solubilized raft and detergent-soluble fractions, and analyzed on 8% SDS-PAGE by anti–SHIP-1 immunoblot (A). An aliquot of the pooled raft or soluble fractions (as above) was loaded on 15% SDS-PAGE and analyzed by Western blotting with antitubulin (B), anti-LAT (C), or peroxidase-conjugated CTB (GM1) (D). An experiment representative of 3 performed is shown.$

Kinetics of CD16-induced SHIP-1 translocation in lipid rafts.

Unstimulated (−) or CD16-stimulated cultured NK cells (3 × 10⁸/sample) were fractionated by sucrose gradient centrifugation as described in “Materials and methods.” SHIP-1 immunoprecipitates were obtained from equal amounts of proteins from solubilized raft and detergent-soluble fractions, and analyzed on 8% SDS-PAGE by anti–SHIP-1 immunoblot (A). An aliquot of the pooled raft or soluble fractions (as above) was loaded on 15% SDS-PAGE and analyzed by Western blotting with antitubulin (B), anti-LAT (C), or peroxidase-conjugated CTB (GM1) (D). An experiment representative of 3 performed is shown.

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