SHIP-1 and GM1 colocalization on CD16 engagement by reverse ADCC.

Alexa Fluor 594–conjugated CTB-labeled NK cells were left untreated (A-C) or pretreated with anti-CD16 (G-I) or anti–MHC class I mAb (D-F), and incubated with Alexa Fluor 594–conjugated CTB-labeled P815 target cells at 37°C for 3 minutes (D-I). After fixing, cells were stained with anti-SHIP mAb and FITC-labeled GAM. Representative examples of isolated NK and P815 cells or NK/P815 conjugates from 3 separate experiments are shown. Effector (e) and target (t) cells were identified on the basis of cell size. Original magnifications × 600.