CD16 receptor and SHIP-1 colocalize after receptor engagement by reverse ADCC.

FITCconjugated anti-CD16– or anti-CD56–treated NK cells were directly fixed (A-C and G-I) or incubated with P815 target cells at 37°C for 3 minutes (D-F and J-L) and then fixed. After fixing, cells were stained with anti-SHIP antibody and Texas red–labeled goat antirabbit IgG. Representative examples of isolated NK cells or NK/P815 conjugates from 3 separate experiments are shown. Cells were analyzed by confocal microscopy. Original magnifications × 600.