American Society of Hematology

Fig. 6.

$Fig. 6. CD16 phosphorylated ζ chain associates with SHIP-1 in the raft compartment after receptor engagement. / Cultured NK cells (3 × 108) were either unstimulated (−) or stimulated with CD16 for 2 minutes. Raft and detergent-soluble fractions were prepared as in Figure 3. The ζ chain (A-B) or SHIP-1 (C-G) immunoprecipitates were prepared from equal amounts of proteins from solubilized raft and detergent-soluble fractions, and analyzed by immunoblot with anti-ζ or anti-pTyr mAb. After stripping of the bound antibodies, the membrane corresponding to panels C and D was reprobed with anti-SHIP or anti-shc antibody, respectively. An experiment representative of 3 performed is shown.$

CD16 phosphorylated ζ chain associates with SHIP-1 in the raft compartment after receptor engagement.

Cultured NK cells (3 × 10⁸) were either unstimulated (−) or stimulated with CD16 for 2 minutes. Raft and detergent-soluble fractions were prepared as in Figure 3. The ζ chain (A-B) or SHIP-1 (C-G) immunoprecipitates were prepared from equal amounts of proteins from solubilized raft and detergent-soluble fractions, and analyzed by immunoblot with anti-ζ or anti-pTyr mAb. After stripping of the bound antibodies, the membrane corresponding to panels C and D was reprobed with anti-SHIP or anti-shc antibody, respectively. An experiment representative of 3 performed is shown.

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