Fig. 2.
Fig. 2. KIR triggering prevents CD3-redirected lysis and CD3-induced membrane raft reorganization. / (A) Cytotoxic activity of 4D4 clone was assessed against anti-CD3–coated P815 murine cells. CTL 4D4 was added at a ratio of 5:1 to target in absence (anti-CD3) or in presence of 10 μg/mL of anti-CD158a (+ anti-CD158) or anti–HLA-DR mAbs (+ anti–HLA-DR). (B) Membrane reorganization of CTL clone 4D4 was analyzed by confocal microscopy after stimulation for 15 minutes at 37°C with mAb-coated beads. CTL membrane was labeled with fluorescent B subunit of Cholera Toxin (FITC–CT-B, green), and after cell permeabilization CTLs were labeled with anti-Ptyr mAb revealed by Cy5-labeled goat antimouse IgG (red). An overlay compacted image of serial optical sections is shown on left side panels (i,iii,v), the green color has been removed on right panel (ii,iv,vi; original magnification × 63).

KIR triggering prevents CD3-redirected lysis and CD3-induced membrane raft reorganization.

(A) Cytotoxic activity of 4D4 clone was assessed against anti-CD3–coated P815 murine cells. CTL 4D4 was added at a ratio of 5:1 to target in absence (anti-CD3) or in presence of 10 μg/mL of anti-CD158a (+ anti-CD158) or anti–HLA-DR mAbs (+ anti–HLA-DR). (B) Membrane reorganization of CTL clone 4D4 was analyzed by confocal microscopy after stimulation for 15 minutes at 37°C with mAb-coated beads. CTL membrane was labeled with fluorescent B subunit of Cholera Toxin (FITC–CT-B, green), and after cell permeabilization CTLs were labeled with anti-Ptyr mAb revealed by Cy5-labeled goat antimouse IgG (red). An overlay compacted image of serial optical sections is shown on left side panels (i,iii,v), the green color has been removed on right panel (ii,iv,vi; original magnification × 63).

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