Normal expression of ATM protein in L-4.

(A) The levels of ATM protein present in the lysates from the indicated cell lines were determined by Western blotting using anti-ATM antibody32 and normalization with an α-tubulin antibody. Lanes 1 to 4 are LCLs from patients L-2, L-4, L-3, and L-1, respectively. Lane 5 is a normal control LCL (LCL-wt1). (B) The histograms were obtained by the densitometric analysis of the Western blot autoradiographs, after correction of each lane for α-tubulin content. Values are normalized to those of the normal control LCL-wt (= 1). Although not shown, ATM protein levels in L-5, L-6, and L-7 were comparable to those of LCL-wt1 and of another control (LCL-wt2). Data were expressed as the mean (± SE) of 3 independent experiments.