Western blot detection of TIMP-3 protein in conditioned medium from NXS2 or B16F10 tumor cells transduced with MSCV-TIMP-3-I-GFP or MSCV-I-GFP.

Reduced protein bands in both unglycosylated (24 kDa) and glycosylated (30 kDa) forms are seen. TIMP-3 expression from NXS2 cells FACS-sorted for high and low GFP expression is shown. Recombinant TIMP-3 (100 ng) was placed in the first lane.