Comparable efficiency of pDCs and CD11c⁺ DCs to stimulate an influenza MP–specific CD8⁺ T-cell clone.

Flu16, an HLA-A*0201–restricted influenza MP_(58-66)–specific CD8⁺ T-cell clone, was cocultured with HLA-A*0201⁺ pDCs or CD11c⁺ DCs infected by influenza virus or pulsed with influenza MP_(58-66) peptide. (A) After 24 hours, IFN-γ in the coculture supernatants was measured by ELISA. (B) After 3 days,³H-thymidine was added for 12 hours to measure proliferation. CPM indicates counts per minute.