Comparison of light cell formation during oxy/deoxy cycling versus oxy circulation.

Five experiments were performed in buffer A to determine the effect of oxygenated circulation (ie, without oxy/deoxy cycling) on low-density cell formation. Fast oxy/deoxy cycling produced a significant increase in light cell formation (ρ < 1.087 g/mL) compared with the light cell generation by oxygenated circulation and oxy incubation. There was no significant difference in light cell generation between the oxy circulated cells and the oxy incubated cells (P > .4).