American Society of Hematology

Fig. 9.

$Fig. 9. Increased PS externalization of low-density cells in the presence of calcium. / (A) PS externalization (binding of annexin V–FITC) was measured by flow cytometry in unfractionated, dense, and rehydrated SSRBCs. (B) (C) PS externalization was determined in all density fractions after oxy/deoxy cycling or oxy (37°C) incubation in the presence (panel B) and absence (panel C) of calcium. Generated light cells (F1 and F2) had a higher percentage of PS+ cells than the original F5 cells. The presence of Ca++ greatly enhanced PS externalization in both the oxy incubated and cycled samples.$

Increased PS externalization of low-density cells in the presence of calcium.

(A) PS externalization (binding of annexin V–FITC) was measured by flow cytometry in unfractionated, dense, and rehydrated SSRBCs. (B) (C) PS externalization was determined in all density fractions after oxy/deoxy cycling or oxy (37°C) incubation in the presence (panel B) and absence (panel C) of calcium. Generated light cells (F1 and F2) had a higher percentage of PS⁺ cells than the original F5 cells. The presence of Ca⁺⁺ greatly enhanced PS externalization in both the oxy incubated and cycled samples.

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