American Society of Hematology

Fig. 1.

$Fig. 1. Expression of AID mRNA in human germinal center B lymphocytes. / Total human B cells were fractionated by immunoaffinity purification into naive B cells (CD19+CD38−IgD+), naive germinal center cells (CD19+CD38+IgD+), germinal center cells (CD19+CD38+IgD−), memory cells (CD19+CD38−IgD−), centroblasts (CD19+CD38+CD44−), and centrocytes (CD19+CD38+CD44+). cDNA pools were synthesized from total RNA, and the cDNAs of AID (nt 328-520) and β-actin (nt 391-660) were amplified by PCR. The PCR products were separated by agarose gel electrophoresis and hybridized with internal radiolabeled oligonucleotides.$

Expression of AID mRNA in human germinal center B lymphocytes.

Total human B cells were fractionated by immunoaffinity purification into naive B cells (CD19⁺CD38⁻IgD⁺), naive germinal center cells (CD19⁺CD38⁺IgD⁺), germinal center cells (CD19⁺CD38⁺IgD⁻), memory cells (CD19⁺CD38⁻IgD⁻), centroblasts (CD19⁺CD38⁺CD44⁻), and centrocytes (CD19⁺CD38⁺CD44⁺). cDNA pools were synthesized from total RNA, and the cDNAs of AID (nt 328-520) and β-actin (nt 391-660) were amplified by PCR. The PCR products were separated by agarose gel electrophoresis and hybridized with internal radiolabeled oligonucleotides.

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