Alternative splicing of AID mRNA in human non-Hodgkin lymphoma.

Full-length AID mRNA (nt 11-720) was amplified by PCR from cDNA pools of human NHL, and the PCR products were hybridized with an internal oligonucleotide (A, upper panel). AID splice variants sv1 and sv2 were amplified by half-nested PCR using a specific oligonucleotide (AID14) complementary to the neo-exon in intron 3 (A, lower panel). The primers used for the RT-PCR are indicated with their respective numbers. The amino acid sequence of the 4 different splice variants of the human AID gene is shown (B). The shaded boxes mark the amino acid residues aberrant to the full-length sequence; the asterisks indicate the deleted amino acid residues of the splice variants.