Fig. 5.
Ribonuclease protection for AID mRNA in human non-Hodgkin lymphoma.
Total RNA (10 μg) from human NHL was hybridized with α–32P-labeled AID antisense RNA probe (nt 232-10). After RNA digestion the protected AID antisense RNA was analyzed on a denaturing polyacrylamide sequencing gel. As an internal standard, ribonuclease protection was performed for β-actin mRNA (lower panel). Yeast RNA (yeast) was used as a negative control, and undigested antisense RNA probe was run aside.
