PF-4 inhibits FGF2-induced ERK activation.

(A) Effect of PF-4 on ERK phosphorylation. Serum-deprived ACE cells were incubated for 10 minutes with FGF2 (10 ng/mL) and various concentrations of PF-4 (2 μg/mL to 10 μg/mL). (B) Effect of PF-4 on ERK phosphorylation over time. Serum-deprived ACE cells were incubated with FGF2 (10 ng/mL) in the presence or absence of PF-4 (10 μg/mL) for various lengths of time. Cell lysates were analyzed by Western blotting, using polyclonal antibodies against ERK-P and total ERK. (C) Effect of FGF2 in the presence (dotted line) or absence (plain line) of PF-4. Autoluminograms were scanned with a laser densitometer. Results are representative of 3 independent experiments.