PF-4 selectively inhibits FGF2-induced ERK phosphorylation in CHOm and CHOm-FGF-R1 cells.

(A) Effects of FGF2 on ERK (left panel) and Akt (right panel) phosphorylation in parental CHOm and CHOm-FGF-R1. Serum-deprived CHOm and CHOm-FGF-R1 cells were stimulated for 10 minutes with FGF2 (10 ng/mL). (B) Effects of PF-4 on FGF2-induced ERK and Akt phosphorylation in parental CHOm and CHOm-FGF-R1. Serum-deprived CHOm (left) and CHOm-FGF-R1 (right) cells were stimulated for 10 minutes with FGF2 (10 ng/mL) and various concentrations of PF-4 (1 μg/mL to 10 μg/mL). Cell lysates were analyzed by Western blotting, using polyclonal antibodies against phosphorylated Akt and phosphorylated ERK or total ERK. Results are representative of 3 experiments.