Fig. 4.
Fig. 4. Adhesion of αIIbβ3 mutants to immobilized fibrinogen. / Wild-type αIIb or each mutant αIIb cDNA was transiently cotransfected with wild-type β3 cDNA into 293 cells. Two days after transfection, wild-type or mutant αIIbβ3-transfected cells (1 × 106 cells/well) were incubated with immobilized fibrinogen (FBG) in the presence of 50 μM cyclo(RGDfV) (αvβ3-specific antagonist) at 37°C. After washing with PBS, adherent cells were quantified with a colorimetric reaction using endogenous cellular acid phosphatase activity. Data represent the mean ± SD of triplicate measures of optical density at 415 nm. Statistical analysis (2-tailed P values for paired sample) was performed between 293 cells expressing wild-type αIIbβ3 and those expressing each mutant αIIbβ3 (**P < .01; *P < .05).

Adhesion of αIIbβ3 mutants to immobilized fibrinogen.

Wild-type αIIb or each mutant αIIb cDNA was transiently cotransfected with wild-type β3 cDNA into 293 cells. Two days after transfection, wild-type or mutant αIIbβ3-transfected cells (1 × 106 cells/well) were incubated with immobilized fibrinogen (FBG) in the presence of 50 μM cyclo(RGDfV) (αvβ3-specific antagonist) at 37°C. After washing with PBS, adherent cells were quantified with a colorimetric reaction using endogenous cellular acid phosphatase activity. Data represent the mean ± SD of triplicate measures of optical density at 415 nm. Statistical analysis (2-tailed P values for paired sample) was performed between 293 cells expressing wild-type αIIbβ3 and those expressing each mutant αIIbβ3 (**P < .01; *P < .05).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal